Fig. 5: Activities of the TCA cycle enzymes. | Communications Biology

Fig. 5: Activities of the TCA cycle enzymes.

From: TCA cycle enhancement and uptake of monomeric substrates support growth of marine Roseobacter at low temperature

Fig. 5

a Activities of low-temperature-induced enzymes extracted from cultures at 5 °C and measured at 5 and 25 °C. b Activities of cold-induced (derived from cultures at 5 °C) and temperate-induced (derived from cultures at 25 °C) enzymes measured at 25 °C. One unit of IDH was defined as the amount of enzyme that generates 1.0 μmole of NADPH per minute at pH 8.0 at 5 °C or 25 °C, one unit of α-KGDH was defined as the amount of enzyme that generates 1.0 μmole of NADH per minute at pH 7.5 at 5 °C or 25 °C, one unit of SCS was defined as the amount of enzyme that generates 1.0 mmole of NADH per minute at pH 7.4 at 5 °C or 25 °C, and one unit of SDH was defined as the amount of enzyme that generates 1.0 μmole of DCIP per minute at pH 7.2 at 5 °C or 25 °C. The experiments were performed in triplicate and the average values are given. Error bars represent standard deviations. Significant differences between the two datasets are indicated by asterisks (*p < 0.05; **p < 0.005).

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