Table 1 List of DNA constructs used for experiments in organotypic hippocampal slices.

From: Aion is a bistable anion-conducting channelrhodopsin that provides temporally extended and reversible neuronal silencing

DNA construct

Concentration (ng/µl)

hSyn-PhobosCADC-citrine (Aion)

20

hSyn-Aion-citrine-Kv2.1 (somAion)

20

hSyn-Aion-ts-citrine-ER

5

hSyn-PhobosCADN-citrine

20

hSyn-PhobosCADS-citrine

20

hSyn-PhobosCADH-citrine

20

hSyn-GtACR1CA-citrine

5

hSyn-mCerulean

50

hSyn-citrine

20

  1. Plasmids were used for single-cell electroporation of CA1 neurons at the indicated concentrations.
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