Fig. 3: Inhibition of mTORC1 decreased eNOS expression. | Communications Biology

Fig. 3: Inhibition of mTORC1 decreased eNOS expression.

From: mTOR contributes to endothelium-dependent vasorelaxation by promoting eNOS expression and preventing eNOS uncoupling

Fig. 3

a, b HAEC were treated with 1 nM rapa for 1 h prior to western blot analysis and quantification of eNOS protein (n = 3, a) as well as the ratio of phosphorylated Ser1177 or Thr495 to total eNOS (b). c HAEC were transfected with siRNA followed by western blotting and quantification of eNOS expression (n = 3). d, e HAEC were treated with 1 nM rapa for 1 h (d) or transfected with siRNA prior to quantitative PCR (n = 5–7). f At 48 h, post transfection with siRNA against RPS6KB1, HAEC were submitted to western blot analysis, quantification of eNOS expression (n = 3), and real-time PCR (n = 6). g HAEC were transfected with pcDNA3.1 (sham) or pcDNA3.1 EIF4EBP-1 plasmids followed by western blot analysis, quantification of eNOS expression (n = 3) and quantitative PCR (n = 6). Error bars correspond to standard error of the mean (SEM). *p < 0.05; **p < 0.01; ***p < 0.001 vs. ctrl or sictrl or sham; one sample t test (a, b, d, f, g) or repeated measures (RM) one-way ANOVA with Dunnett’s test (c, e).

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