Fig. 4: p70S6K downstream of mTORC1 contributed to eNOS transcription through translational regulation of KLF2 expression. | Communications Biology

Fig. 4: p70S6K downstream of mTORC1 contributed to eNOS transcription through translational regulation of KLF2 expression.

From: mTOR contributes to endothelium-dependent vasorelaxation by promoting eNOS expression and preventing eNOS uncoupling

Fig. 4

a, b HAEC were treated with 1 nM rapa or transfected with siRNA prior to Western blot analysis and quantification of KLF2 expression (n = 3–4). c, d HAEC were transfected with KLF2-targeting siRNA followed by Western blotting (c) and quantitative PCR (n = 4, d). e At 48 h post-transfection with siRNA against KLF2, relative NO level of the culture medium was measured with the colorimetric assay (n = 5). f HAEC were treated with 1 nM rapa for 1 h, prior to quantitative PCR to measure mRNA (n = 3). g, h HAEC were transfected with siRNA against RPS6KB1 followed by quantitative PCR (n = 7, g) or western blotting (n = 4, h) to quantify KLF2 expression. **p < 0.01; ***p < 0.001 vs. sictrl or ctrl; one-sample t test (a, d, f, g, h) or RM one-way ANOVA with Dunnett’s test (b) or unpaired two-tailed t test (e).

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