Fig. 6: Crosstalk between MAPK (p38 and JNK) activation and Nox2 upregulation contributed to excessive ROS production in mTORC2-inhibited HAEC. | Communications Biology

Fig. 6: Crosstalk between MAPK (p38 and JNK) activation and Nox2 upregulation contributed to excessive ROS production in mTORC2-inhibited HAEC.

From: mTOR contributes to endothelium-dependent vasorelaxation by promoting eNOS expression and preventing eNOS uncoupling

Fig. 6

a After treatment with 10 nM torin or 1 nM rapa for 1 h, HAEC were submitted to western blot analysis. b–d HAEC were pretreated with SB 203580 (SB, 20 µM), SP 600125 (SP, 20 µM) or U0126 (5 µM) for 1 h before incubation with or without 10 nM torin for 1 h prior to DHE staining (n = 5, b), NO assay (n = 3–4, c) or quantitative PCR (n = 4–5, d). e HAEC were treated with SB (20 µM) or SP (20 µM) for 1 h before incubation with 10 nM torin for 1 h prior to Western blotting and quantification of Nox2 expression. f After pretreatment with Apo (20 µM) for 1 h, HAEC were incubated with or without 10 nM torin for 1 h before western blot analysis and quantification of JNK and p38 phosphorylation. *p < 0.05 vs. untreated ctrl; #p < 0.05 vs. torin-treated ctrl; RM one-way ANOVA with Dunnett’s test (b–d) or Tukey’s test (e, f).

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