Fig. 7: Fusobacterium spp. modulate metabolites associated with hydrogen sulfide production and oxidative stress.

a Bubble plots representing enrichment of methionine and cysteine metabolism and glutathione metabolism from metabolomics data derived from 3-D cervical cells infected with BVAB. Bubble sizes are proportional to the enrichment factor and bubble colors indicate significance. b Methionine and cysteine metabolic pathway depicting hydrogen sulfide production (yellow circles). c Glutathione metabolic pathway that participates in the oxidative stress response. Asterisks next to metabolites are also represented in the heatmap. d Heatmap representing statistically different (p < 0.05) metabolites corresponding to methionine and cysteine metabolism and glutathione metabolism that were differentially accumulated (orange and yellow shading) or depleted (blue shading) during infection with Fusobacterium species. Fold change difference between infection with and mock-infected controls are indicated as numerical values within the heat map cells. A minimum of n = 3 independent replicates were performed and measured for each condition.