Fig. 3: Benchmarking of MeasureIce with electron tomography.
From: MeasureIce: accessible on-the-fly measurement of ice thickness in cryo-electron microscopy
For each hole particles at the extremities in z were manually picked from the grid and a surface fitted to these points (a), the height difference between these two surfaces is taken to be the estimated thickness and this is plotted in (b) alongside the same grid hole measured using the MeasureIce approach. Shown in (c) is a comparison of ice thickness as measured by tomography and MeasureIce for four different grid holes on an apo-ferritin containing carbon Quantifoil grid. Each datapoint in (c) is a single pixel in the MeasureIce thickness map. The MeasureIce thickness map for two of the tomograms is inset in (d).
