Table 1 Comparison of apoferritin data collection and reconstruction parameters between this paper and that in Naydenova et al.1.
From: MeasureIce: accessible on-the-fly measurement of ice thickness in cryo-electron microscopy
This paper | Naydenova et al.16 | |
|---|---|---|
Grids | Gold Ultrafoil 1.2/1.3 | Functionalized graphene-coated gold Ultrafoil 1.2/1.3 |
Microscope | Titan Krios G4 | Titan Krios |
Accelerating voltage (kV) | 300 | 300 |
Magnification | 120,000 | 120,000 |
Pixel size (Å) | 0.637 | 0.6495 |
Exposure time (s) | 1.52 | 30.0 |
Dose (e/Å2) | 16 | 37 |
Number of Particles used in final refinement | 68,025 | 41,202 |
Gold standard FSC map resolution (Å) | 1.88 | 2.14 |
B-factor (Å2) | −54 | −54 |
