Fig. 3: Coacervation of Pvfp-5β in alkaline environment.
From: Solution structure of recombinant Pvfp-5β reveals insights into mussel adhesion
Phasor analysis of 256 × 256 pixels FLIM measurements on ThT signal of 1 mg/ml Pvfp-5β in 0.1 M Tris-HCl pH 8, 1 M NaCl. a Phasor plot obtained from images of the different species present in the sample. The coordinates g and s correspond to sine and 30 cosine transforms of each point in the phasor plot, respectively. Three distinguishable lifetime distributions, highlighted by colored circular cursors, are evident and lay on a straight line (dashed yellow) connecting two mono-exponential decays phasors with characteristic lifetime of 2.4 ns and 0.4 ns. b, c, d Fluorescence intensity images, from low intensity (blue) to high intensity (red) of coacervate droplets, medium size aggregates and fibril, respectively. e, f, g Lifetime maps: each pixel is colored according to the corresponding color code of the cursor highlighting the lifetime distributions in the phasor plot a).
