Fig. 3: Relative proportion of cell fates following different pharmacological treatments.

Relative percentage of neurons that display no change, clear apoptotic, non-apoptotic necrotic-like cell fate, or high chromatin compaction defined as granulation upon different pharmacological treatments (control n = 460 cells from 9 experiments; staurosporine n = 518 cells from 9 experiments; staurosporine with caspase-3 inhibitor n = 265 cells from 5 experiments; BDM n = 164 cells from 3 experiments; staurosporine with BDM n = 301 cells from 5 experiments). a For most neurons under control condition, no change in nuclear appearance was observable with no significant caspase activation (measured by the intensity of the NucView signal) and no chromatin compaction until the end of 6.5 h live cell experiment (blue). b In staurosporine-treated cultures, many nuclei presented either a strong caspase activation and a decrease in nuclear size (apoptosis in red) or nuclei did not present a decrease in nuclear size but presented an increase in CCP, thus classified as “granulated” (yellow). c Whereas, if staurosporine treatment was combined with the application of the caspase-3 inhibitor Z-DEVD-FMK, only few nuclei presented an increase in caspase activation, but more neurons showed a decrease in nuclear size and a high CCP and edge count at the end of the experiment and were thus classified as necrotic (gray). d Under BDM treatment, an increase in the relative proportion of neuronal nuclei that presented an increase in caspase activation, a decrease in nuclear size and a high CCP and edge count at the end of the experiment, thus classified as necrotic (gray), was observed. e If staurosporine treatment was combined with the application of BDM, the number of neuronal nuclei classified as necrotic (gray) increased, as well as the number of granulated nuclei.