Fig. 2: Hepatocyte-specific response to CYP induction was monitored by Raman microscopy. | Communications Biology

Fig. 2: Hepatocyte-specific response to CYP induction was monitored by Raman microscopy.

From: Label-free chemical imaging of cytochrome P450 activity by Raman microscopy

Fig. 2

a Raman spectra of hepatocytes (red) and biliary cells (gray) without CYP induction. b Immunofluorescence staining of CYP3A4 (green) and CK-19 (red) with nuclear counterstain by DAPI. Hepatocyte cells (HC) are CYP3A4 positive; while biliary cells (BC) are CK-19 positive. Scale bar, 50 µm. Reconstructed Raman images of control hepatocytes (c Ctrl-H), control biliary cells (d Ctrl-B), RIF-treated hepatocytes (e RIF-H), and RIF-treated biliary cells (f RIF-B) show the specific response of hepatocytes to RIF induction. Scale bars, 20 µm. g Average spectra of Ctrl-H (gray), Ctrl-B (light gray), RIF-H (red), and RIF-B (light red).

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