Fig. 5: CYP induction with glycogen depletion.
From: Label-free chemical imaging of cytochrome P450 activity by Raman microscopy
a Hyperspectral Raman images reveal dynamic changes in hepatic functional molecules (cyt b5, glycogen, and CYPs) and general cellular components (cyt c, phenylalanine, and lipids) from D0 to D7. An accumulation of glycogen is observed, whereas treatment of CYP inducer deceases the glycogen amount. Scale bars, 20 µm. b Quantitative analysis of the Raman intensity at 940 cm−1 and 1636 cm−1 shows decreased glycogen with increased CYP after CYP inducer treatment. Box plots illustrate the spread within cell populations (center line, median; box, quartiles; whiskers, 1.5× interquartile range). ***P < 0.001. c Single-cell-based dot plot with normalized Raman intensity at 940 cm−1 (x axis) and 1636 cm−1 (y axis). Each dot indicates one cell. d PAS staining demonstrates the accumulation of glycogen during the 7-day culture and consumption with RIF treatment. Scale bars, 100 µm.
