Fig. 3: Sequence alignment of AlyV, PyAly, A1-II’ and CD2^AlyB.

The three conserved regions in the PL7 family are boxed in red, and two conserved catalytic residues are marked with stars. For PyAly, A1-II’ and CD2^AlyB, the residues involved in substrate binding at subsites +1 to +3 are shaded in red, and the conserved residues in the PL7 family are boxed in black. For PyAly and AlyV, the residues involved in substrate binding at subsites -1 to -3 are shaded in blue.