Fig. 5: Two loops in the “-” section were demonstrated to determine product distribution.

a The positions of two loops (loop1 and loop2) from AlyV and PyAly. loop1AlyV: Pro117-Ala134; loop2AlyV: Val183-Leu210; loop1PyAly: Thr108-Lys117; loop2PyAly: Arg159-Phe170. b Diagram of the constructed chimeric PyAly M1, containing loop1AlyV and loop2AlyV. c Product distributions of M1, M1-1, and M1-2.