Fig. 3: Antibody neutralization of wild type and VOCs.

a Antibody neutralization curves of wild type, Alpha, Beta, and Delta VOC pseudo-typed GFP reporter viral particles. For every mAb, each curve represents inhibition of one VOC as indicated. Antibodies were pre-incubated with viral particles at 8 consecutive 3-fold dilutions starting at 10 µg/mL of antibody. Fluorescence of infected cells was read 72 h post infection. Inhibition percentage was calculated by normalizing to untreated cells. Each experiment was done in triplicate (n = 3). mGO53 was used as an isotype control. Error bars indicate SD. b Infection of Vero-TMPRSS2 cells by wild type (n = 4), Alpha (n = 2), Beta (n = 4), Gamma (n = 4), Delta and Omicron (n = 6) with authentic SARS-CoV-2 and overlayed with carboxymethylcellulose. Values are expressed as the infected surface area of cells and normalized to infected cells without mAb. Viral particles were pre-incubated with 100 µg/mL of antibody for 1 h before addition to the cells. Infected cells were identified at 24 h post infection using SARS-CoV-2 nucleocapsid antibody after cell fixation and permeabilization. Infected cells were quantified using Incucyte S3. mGO53 serves as an isotype control. Error bars indicate SD.