Fig. 6: Histological sections of marmoset tissue immunostained for olfactory marker protein (OMP) or acyl-coenzyme A synthetase medium chain family member-4 (ACSM4).

a Histological sections of the olfactory epithelium of the marmoset. The mucosa consists of an upper layer containing mainly supporting cells (SC), a middle layer of olfactory nerve cells (ON) that display chemoreceptors on the mucosal surface, and a lower layer of basal cells (BC). Nerve bundles consisting of multiple nerve cell axons can be seen in the submucosa. Scale bars: 100 μm. ACSM4, acyl-coenzyme A synthetase medium chain family member-4; HE, hematoxylin-eosin; OMP, olfactory marker protein; W/O Ab, without antibody. b Tissue sections were obtained at four levels (i, ii, iii and iv) for further analysis. c Histological sections of the marmoset nasal cavity immunostained for OMP. The OMP-positive regions are shown in red. OMP-positive regions were seen in both the mucosal layer and as nerve bundles in the submucosal layer. Scale bars: 1 mm. IT inferior turbinate, MT middle turbinate, OMP olfactory marker protein, ST superior turbinate. d Histological sections of the marmoset nasal cavity immunostained for ACSM4. The ACSM4-positive regions are highlighted in red. The ACSM4-positive regions were limited to the mucosal layer and were less extensive than the OMP-positive regions. Scale bars: 1 mm. IT inferior turbinate, MT middle turbinate, OMP olfactory marker protein, ST superior turbinate. e Overlaid magnetic resonance images, tractography images and OMP-stained images. Tractography detected the thicker nerve bundles in the submucosal layer. Scale bars: 1 mm.