Table. 1 List of a several biomolecules directly used in the biochemical experiment, both in vitro and in vivo.
Chemical species | Assay concentration |
|---|---|
CONTROL (only SEC buffer) | See material and methods |
Tris HCl pH = 8.0 | 20 mM |
Tris HCl pH = 8.5 | 20 mM |
Hepes Na pH = 7.0 | 20 mM |
Hepes Na pH = 7.5 | 20 mM |
H2O2 | 100 μM |
FBS | 0.1% v/v |
Trypsine | 0.05 mg/ml |
NaOH | 100 μM |
FeCl2 | 100 μM |
β-mercaptoethanol | 1 mM |
Cysteine | 100 μM |
Glycine | 100 μM |
Glutamate | 100 μM |
Lysine | 100 μM |
Tryptophane | 100 μM |
Tyrosine | 100 μM |
NaCl | 150 mM |
KCl | 150 mM |
MgCl2 | 2.5 mM |
ZnSO4 | 100 μM |
NAD+ | 500 μM |
DMSO | 15% |
Probe 10/11 diluted in SEC buffer | Probe 10/11 = 10 μM |
Probe 10/11 diluted in SEC buffer + ALDH1A1 | ALDH1A1 = 100 μM |
Probe 10/11 diluted in SEC buffer + ALDH1A2 | ALDH1A2 = 100 μM |
Probe 10/11 diluted in SEC buffer + ALDH1A3 | ALDH1A3 = 100 μM |
