Fig. 2: PEDF expression is associated with increased intraperitoneal dissemination of both mouse and human OC cells and with poor prognosis.

a Omental tumor weight, the number of tumor nodules, and the volume of ascites fluid for C57BL/6 J mice at 90 days after injection of ID8G-EV (n = 8 mice) or ID8G-PEDF (n = 7 mice) cells into the peritoneal cavity. Data are means ± SD. b Survival curves for mice injected with ID8G-EV (n = 9) or ID8G-PEDF cells (n = 11). The P value was determined with the log-rank test. c Omental tumor weight, number of tumor nodules, and the volume of ascites fluid for C57BL/6 J mice at 63 days after injection of PEDF^high or PEDF^low clones of ID8G cells into the peritoneal cavity. Data are means ± SD (n = 7 or 8 mice). Data for omental tumor weight and number of tumor nodules were analyzed by Welch’s ANOVA followed by Dunnett’s post hoc test and for ascites by Tukey’s multiple comparisons test. d Survival curves for mice injected with PEDF^high or PEDF^low clones of ID8G cells (n = 4 each). The P value was determined with the log-rank test. e Pearson’s correlation analysis for expression of PEDF mRNA versus survival time of mice inoculated with the corresponding cells. Data are median ± 95% CI. f Omental and intraperitoneal disseminated tumor weight as well as the volume of ascites fluid in nude mice at 27 days after injection SKOV3ip1-EV cells (n = 7 mice) or SKOV3ip1-PEDF cells (n = 6 mice) into the peritoneal cavity. Data are means ± SD. g Survival curves for mice injected with SKOV3ip1-EV or SKOV3ip1-PEDF cells (n = 8 each). The P value was determined with the log-rank test. Data for a and f were analyzed by unpaired t test with Welch’s correction.