Fig. 3: Effects of PEDF on OC cells.

a Anoikis resistance assay for ID8G-EV and ID8G-PEDF cells (n = 5 each) and for SKOV3ip1-EV and SKOV3ip1-PEDF cells (n = 6 each). b Attachment assay for ID8G-EV and ID8G-PEDF cells and for SKOV3ip1-EV and SKOV3ip1-PEDF cells cultured in wells coated with fibronectin (n = 6 each). c, d Cell proliferation assay for ID8G-EV and ID8G-PEDF cells (c) (n = 5 each) and for SKOV3ip1-EV and SKOV3ip1-PEDF cells (d) (n = 6 each). All data are means ± SD for the indicated number of replicates from representative experiments out of a total of three performed. e Omentectomized or sham-operated C57BL/6 J mice were injected i.p. with ID8G-PEDF cells into the peritoneal cavity. f The number of tumor nodules and the volume of ascites fluid for omentectomized (n = 6 mice) or sham-operated (n = 5 mice) C57BL/6 J mice at 86 days after injection of ID8G-PEDF cells into the peritoneal cavity. Data are means ± SD. g C57BL/6 J mice were injected i.p. with ID8G-EV or ID8G-PEDF cells into the peritoneal cavity. At each time point, mice were sacrificed and peritoneal cells were collected for further analysis. h Number of GFP⁺ cells in peritoneal washes at the indicated times after i.p. injection of ID8G-EV or ID8G-PEDF cells (5 × 10⁶) into C57BL/6 J mice (n = 3 or 4 mice for each group at each time point as in g). i Surviving GFP⁺ tumor cells in the peritoneal cavity of C57BL/6 J mice at 5 days after i.p. injection of GO2 sh#1 or sh#2 or corresponding control (sh ct) cells (n = 9 mice per group). All data are means ± SD. Data for a, c, d were analyzed by two-way ANOVA. Data for f, h, i were analyzed by unpaired t test with Welch’s correction.