Fig. 4: Intracellular localization of LLO-lacZ secreted β-gal.
From: Engineered endosymbionts that alter mammalian cell surface marker, cytokine and chemokine expression
The presence of β-gal in the nuclei of host cells was determined by measuring fluorescent signals of anti-β-gal in the nuclei compared to background signals as a ratio (SNR). Fluorescence microscopy (top) and SNR (bottom) of the following: J774A.1 cells with no LLO strain (none), J774A.1 cells incubated with β-gal collected as supernatant from induced LLO-lacZ-NLS (supernatant), J774A.1 cells incubated with uninduced LLO-lacZ-NLS (-mannose), J774A.1 cells incubated with induced LLO-lacZ-no NLS (mannose -NLS) and J774A.1 cells incubated with induced LLO-lacZ-NLS (mannose NLS). Plotted data is mean ± SD from n = 50 random individuals from a representative experiment; ******p < 0.000001. Scale bars = 20 µm.
