Fig. 5: Flow cytometry demonstrated TF strain-mediated shifts of J774A.1 cell marker expression.
From: Engineered endosymbionts that alter mammalian cell surface marker, cytokine and chemokine expression
Flow cytometric analysis revealed changes in CD86 and CD206 induced by expression of transcription factors from the EES (top panel). These data are representative examples, taken as the median value of n = 3. Average CD86 or CD206 median fluorescence intensity (MFI) is compared among all treatments at each time point (lower panel, n = 3); (a) CD206 at 24 h, (b) CD86 at 24 h, (a) CD206 at 48 h, (b) CD86 at 48 h. J774A.1 cells were untreated (none), treated with LPS and IFN-γ (M1+), IL-4 and IL-13 (M2+), B. subtilis strain 168 (168), LLO strain (LLO), LLO-SK with and without mannose (LLO-SK -mannose, LLO-SK + mannose) and LLO-KG with and without mannose (LLO-KG -mannose, LLO-KG + mannose) at 24 and 48 h post initial treatment. Plotted data is mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001, *****p < 0.00001.
