Fig. 6: TF-expressing strains modulated cytokine and chemokine expression in J774A.1 cells.
From: Engineered endosymbionts that alter mammalian cell surface marker, cytokine and chemokine expression
Cytokine and chemokine protein concentrations were assayed by Luminex in untreated J774A.1 cells (none), and in cells treated with LPS and IFN-γ (M1+), IL-4 and IL-13 (M2+), LLO strain (LLO), LLO-SK with and without mannose (LLO-SK -mannose, LLO-SK + mannose) and LLO-KG with and without mannose (LLO-KG -mannose, LLO-KG + mannose) at 24 and 48 h-post initial treatment; (a) IL-10 at 48 h, (b) IL-12p40 at 24 h, (c) IL-12p40 at 48 h, (d) TNF-alpha at 24 h, (e) G-CSF at 48 h, and IL-1beta at 48 h. The most prominent protein changes are shown; 14 of the 17 proteins in the panel revealed gene-specific distinctions between the LLO-SK and LLO-KG strains. Plotted data is mean ± SD from n = 3 biological replicates; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ******p < 0.000001. Significance shown is comparing LLO treatment to all other treatments.
