Fig. 2: Acetylated S1 preferentially recruits different subpools of mRNAs to ribosomes.

a Schematic of the ribosome-nascent-chain-complex sequence (RNC-Seq) of acetyl-mimetic S1 (K411Q/K464Q) or WT-S1-containing ribosomes. b Scatterplots for differentially expressed genes (up, red; down, blue.) in KQ/WT- RNC-Seq (p < 0.05). c KEGG pathway-based cluster analysis of KQ/WT-RNC-Seq upregulated genes and downregulated genes from b. d Significantly enriched KEGG pathways (P < 0.001) among Up-set or Down-set transcripts in KQ/WT-RNC-Seq. The key KEGG pathways are shown, and the number of associated genes in each KEGG pathway is shown in parentheses.