Fig. 1: The strategy of the whole Genome Analysis and Genome Editing (GAGE). | Communications Biology

Fig. 1: The strategy of the whole Genome Analysis and Genome Editing (GAGE).

From: GAGE is a method for identification of plant species based on whole genome analysis and genome editing

Fig. 1

The fluorophore (6-Carboxyfluorescein, FAM) is linked to the quencher (Black Hole Quencher-1, BHQ) through ten dAs (ssDNA) and is initially quenched. After breaking away from BHQ, FAM absorbs light at its excitation wavelength and emits fluorescence. PAM: protospacer adjacent motif; The whole genome: The schematic genome draft was cited from the reference32; Targets library: The gather of target sequences; Target sequence: The sequence with a nearby PAM; crRNA: CRISPR RNA; ssDNA reporter: single-stranded DNA reporter (5’-FAM-AAAAAAAAAA-BHQ-3’, Poly_A_FQ).

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