Fig. 5: Application of GAGE in plants from different classes. | Communications Biology

Fig. 5: Application of GAGE in plants from different classes.

From: GAGE is a method for identification of plant species based on whole genome analysis and genome editing

Fig. 5

a Statistics of Targets in different classification derived from the four genomes. Genome: Targets located in the whole genome; Unannotated regions: Targets located in the unannotated regions of genome; Coding genes: Targets located in coding genes; CDS: Targets located in CDS. b Result of employing GAGE in plants from different classes. The DNA substrates and crRNA of every group were as follow: Rc: ITS2 fragments of R. communis + Rc_crRNA; Si: ITS2 fragments of Set. italica + Si_crRNA; Gb: ITS2 fragments of G. biloba + Gb_crRNA; As: ITS2 fragments of A. spinulosa + As_crRNA; St: ITS2 fragments of Sel. tamariscina + St_crRNA and CK (negative control): nuclease-free water + Cs_crRNA. All plots data represent means +/− standard deviation (SD) from three independent replicates.

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