Fig. 8: DHA increases the membrane damage capacity of colistin. | Communications Biology

Fig. 8: DHA increases the membrane damage capacity of colistin.

From: Commercialized artemisinin derivatives combined with colistin protect against critical Gram-negative bacterial infection

Fig. 8

a, b DHA addition decreased the expression of unknown proteins (outlined in red) in the bacterial precipitation of E. coli W3110 (pUC19-mcr-1) at 3 and 6 h. Samples were loaded on SDS–PAGE gels and analyzed by staining with Coomassie brilliant blue. c, d Detection of intracellular ATP levels in E. coli W3110 (pUC19-mcr-1) and E. coli W3110 (pUC19). e, f The permeability of the inner membrane of E. coli W3110 (pUC19-mcr-1) treated with DHA (0, 8–64 μg/mL), colistin (0, 1–4 μg/mL) or colistin (0, 1–4 μg/mL) in combination with DHA (32 μg/mL) for 30 min, probed with 5 μM PI. g–i Zeta potential of E. coli W3110 (pUC19-mcr-1) and E. coli W3110 (pUC19) treated with DHA (0, 8-64 μg/mL), colistin (0, 1–16 μg/mL) or colistin (0, 1–4 μg/mL) in combination with DHA (32 μg/mL) were performed. The experiments were repeated three times independently. *P < 0.05. **P < 0.01.

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