Fig. 2: LI14 peptide is a potent broad-spectrum bactericidal agent.
From: The antimicrobial peptide LI14 combats multidrug-resistant bacterial infections
a Growth inhibition of four strains involves S. aureus ATCC 29213, MRSA T144, E. coli ATCC 25922, and E. coli B2 after incubation with various concentrations of LI14 peptide for 18 h at 37 °C. b–e Killing of S. aureus ATCC 29213 (b), MRSA T144 (c), E. coli ATCC 25922 (d), and E. coli B2 (e) in MHB broth in the presence of difference concentrations of LI14 peptide after incubation for 0.5 to 12 h at 37 °C. The initial cell density of ~105 CFUs/mL for Gram-positive bacteria, ~106 CFUs/mL for Gram-negative bacteria. f Killing of E. coli B2 in PBS (nutrient depletion) treated by the varying concentrations of LI14 after incubation for 0.5–6 h at 37 °C. g Biofilm inhibition ability of sub-inhibitory concentrations of LI14 peptide against two tested strains, MRSA T144 and E. coli B2. One-way ANONA was used to determine the statistical significance (**P < 0.01 and ****P < 0.0001). h, i LI14 efficiently eradicates established biofilms (h) and persister cells (i) of MRSA T144 and E. coli B2. Experiments were conducted with three biological replicates and data were shown as mean ± SD.
