Table 1 Antimicrobial spectrum of engineered LI peptides (MIC, μg/mL).

S. aureus ATCC 29213

>128

8

32

<0.0625

16

0.125

MRSA T144

>128

4

32

1

16

0.25

MRSA 1518

>128

64

32

0.25

16

0.25

MRSA 1530

>128

64

32

0.25

16

1

S. aureus 215 (cfr + LZD^R)

64

1

16

1

16

0.25

S. aureus G16 (RIF^R)

32

1

16

2

1

<0.0625

E. faecalis A4 (VRE, VanA)

>128

8

32

>64

64

<0.0625

E. faecalis 1F-1

>128

64

64

8

>64

16

E. faecium 5F-10

>128

64

64

8

>64

<0.0625

E. coli ATCC 25922

>128

8

64

0.03

0.5

0.125

E. coli B2 (bla_NDM-5 + mcr-1)

>128

4

64

32

8

2

E. coli C3 (bla_NDM-1)

>128

4

32

8

<0.125

2

E. coli G6 (bla_NDM-5)

>128

4

16

64

0.5

2

E. coli G92 (mcr-1)

>128

8

32

<0.125

4

4

E. coli CP131 (mcr-3)

>128

4

16

<0.125

2

2

E. coli B3-1 (tet(X4))

>128

8

32

<0.0625

0.0625

32

E. coli 1F28 (tet(X4))

>128

8

32

0.25

0.125

16

S. enteritidis ATCC 13076

>128

8

32

<0.0625

0.25

0.125

A. baumannii ATCC 19609

>128

8

32

<0.0625

0.125

0.25

A. baumannii C222 (tet(X6))

>128

16

32

<0.125

<0.125

64

P. aeruginosa PA14

>128

16

32

32

1

<0.0625

K. pneumoniae ATCC 700603

>128

32

64

<0.0625

1

0.5

K. pneumoniae D120 (mcr-8)

>128

32

32

<0.0625

4

0.25

P. cibarius HNCF44W (bla_NDM-1 + tet(X6))

>128

8

32

>16

>256

64

E. coli DH5α (PUC19)

>128

2

16

<0.125

<0.125

1

E. coli DH5α (PUC19-tet(X4))

>128

2

16

<0.125

<0.125

64

E. coli DH5α (PUC19-bla_NDM-1)

>128

2

16

32

<0.125

1

E. coli DH5α (PUC19-bla_NDM-5)

>128

4

16

64

<0.125

1

E. coli DH5α (PUC19-bla_NDM-9)

>128

2

16

64

<0.125

1

E. coli DH5α (PUC19-mcr-1)

>128

2

16

<0.125

2

1