Fig. 4: MLPL1 from Nepeta mussinii enables 7-deoxyloganic acid production in N. benthamiana. | Communications Biology

Fig. 4: MLPL1 from Nepeta mussinii enables 7-deoxyloganic acid production in N. benthamiana.

From: Reconstitution of monoterpene indole alkaloid biosynthesis in genome engineered Nicotiana benthamiana

Fig. 4

Transient expression of all pathway enzymes plus MLPL produces a peak of 359.1342 m/z which matches the mass and retention time of a 7-deoxyloganic acid (7-DLA) standard. The reconstituted pathway without DLGT also produces a 359.1343 m/z peak but at a different retention time, likely due to endogenous glycosyltransferases of N. benthamiana producing the glucose ester of 7-deoxyloganetic acid. DXS, 1-deoxy-D-xylulose 5-phosphate synthase; GPPS, geranyl diphosphate synthase; GES, geraniol synthase; G8H, geraniol 8-oxidase; GOR, 8-hydroxygeraniol oxidoreductase; ISY, iridoid synthase; MLPL, major latex protein–like; IO, iridoid oxidase; 7-DLGT, 7-deoxyloganetic acid glucosyltransferase.

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