Fig. 5: Reconstitution of strictosidine biosynthesis in N. benthamiana. | Communications Biology

Fig. 5: Reconstitution of strictosidine biosynthesis in N. benthamiana.

From: Reconstitution of monoterpene indole alkaloid biosynthesis in genome engineered Nicotiana benthamiana

Fig. 5

a Quantification of intermediates and final product strictosidine by UPLC/MS analysis. b Absence of MLPL reduces strictosidine production >60 fold while supplementation of GPPS improves yield ~5 fold. c the total ion chromatogram of leaf tissue infiltrated with the entire pathway to strictosidine (including DXS, GPPS, and MLPL). The peak at 4.09 min retention time in the total ion chromatogram (TIC) and extracted ion chromatogram (EIC) at 531.2336 m/z matches a strictosidine standard. N.D., not detected; 7-DLH, 7-deoxyloganic acid hydroxylase; LAMT, loganic acid O-methyltransferase; SLS, secologanin synthase; TDC, tryptophan decarboxylase; STR, strictosidine synthase. Values and error bars represent the mean and the standard error of n = 3 or n = 6 biological replicates (independent leaf samples).

Back to article page