Table 1 Lines of N. benthamiana with Cas9-induced mutations in UGTs.

From: Reconstitution of monoterpene indole alkaloid biosynthesis in genome engineered Nicotiana benthamiana

 

T1 Line

Plasmid

Group

NbUGT

T-DNA

Progeny of T0 transgenic plants regenerated in tissue culture

0720-06-01

pEPQDPKN0720

D

73A24* 73A25* 73Q2 73A32

−

0720-06-03

D

73A24* 73A25* 73Q2 73A32

+

0724-37-06

pEPQDPKN0724

E

72AY1*

72AX1

72B34

72B35

72B58

−

0724-22a-06

E

72AY1*

72AX1

72B34

72B35

72B58

+

0361-34-05

pEPQDPKN0361

G

85A73* 85A74 86A104

−

0361-34-08

G

85A73* 85A74 86A104

+

Progeny of Cas9 transgenic plants infiltrated with mobile sgRNAs

NbCas9-0777B-01

pEPQDKN0777

C,E,L

90K1 71AT3 74P7

n/a

NbCas9-0778B-05

pEPQDKN0778

D,E,O

73E22 71A56 93T3

n/a

NbCas9-0779A-01

pEPQDKN0779

D,E,L

73BY1 71A57 74N6

n/a

NbCas9-0780B-01

pEPQDKN0780

A,L,L

94E7 74N4 74T6

n/a

NbCas9-0781B-03

pEPQDKN0781

D,D,E,G

73A24* 73A25* 72AY1* 85A73*

n/a

  1. Asterisks indicate that activity on geraniol has previously been reported62. NbUGTs in italics were wild-type or contained small in-frame deletions unlikely to inactivate the enzyme. T-DNA represents the presence (+) or absence (−) of the T-DNA delivered in this experiment.
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