Fig. 4: Gene ontology and GeneMANIA analysis provide further mechanistic insight into the molecular landscape of appetite and aversive engram regulation.

a Gene Enrichment Set Analysis of the upregulated pathways in aversive engrams using Gene Ontology (GO) module. The size of the dot represents the gene count, and the color of the dot indicates the FDR. The pathway with an FDR-adjusted P value smaller than 0.25 is considered as significantly enriched. b GO analysis of downregulated pathways in aversive vCA1 cells. c GO analysis of upregulated pathways in appetitive vCA1 cells. d GO analysis of downregulated pathways in appetitive vCA1 cells. GeneMANIA network of the top 20 upregulated and downregulated genes in aversive and appetitive vCA1 cells, respectively. Genes with red nodes are upregulated e, or downregulated f, in the aversive vCA1 cells compared to the neutral sample, and genes with green nodes are upregulated (g) or downregulated (h) in the appetitive vCA1 compared to the neutral sample. The grey nodes represent the genes interacting with these 20 differentially expressed genes. The shared protein domains supported by InterPro domain databases within each network were labeled in red for aversive vCA1 cells and in green for appetitive vCA1 cells. The detailed description for each shared protein domain is listed in Supplementary Table 4. The interaction network categories between these genes are annotated in the legend next to panel g.