Fig. 2: Tob1 deficiency enhances IFN-β expression. | Communications Biology

Fig. 2: Tob1 deficiency enhances IFN-β expression.

From: TOB1 attenuates IRF3-directed antiviral responses by recruiting HDAC8 to specifically suppress IFN-β expression

Fig. 2

a Quantitative real-time RT-PCR analysis of IFN-β, TNF-α, and IL-6 mRNA expression in peritoneal macrophages (PMs) isolated from Tob1+/+ or Tob1−/− mice and then infected with VSV. b Quantitative real-time RT-PCR analysis of IFN-β mRNA expression in PMs isolated from Tob1+/+ or Tob1−/− mice and then exposed to lipopolysaccharide (LPS) or infected with SeV or HSV-1. c Enzyme-linked immunosorbent assay (ELISA) of IFN-β secretion in the supernatants of PMs from Tob1+/+ or Tob1−/− mice after treatment as indicated. d–f Western blot analysis of STAT1 phosphorylation in PMs isolated from Tob1+/+ or Tob1−/− mice and then exposed to SeV (d), LPS (e), or VSV (f). g Quantitative real-time RT-PCR analysis of Ccl5, Isg15, Isg56, and Cxcl10 mRNA expression PMs isolated from Tob1+/+ or Tob1−/− mice and then infected with VSV. All data are shown as the means ± SD. Significance was determined by unpaired two-tailed Student’s t-test: *p < 0.05, **p < 0.01, ***p < 0.001, ns, p > 0.05. Data are shown as a representative result from three independent experiments. Data are shown as a representative result from three independent experiments.

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