Fig. 3: TOB1 deficiency enhance innate immune responses against VSV in vivo. | Communications Biology

Fig. 3: TOB1 deficiency enhance innate immune responses against VSV in vivo.

From: TOB1 attenuates IRF3-directed antiviral responses by recruiting HDAC8 to specifically suppress IFN-β expression

Fig. 3

a Quantitative real-time RT-PCR analysis of VSV mRNA expression in peritoneal macrophages (PMs) isolated from Tob1+/+ or Tob1−/− mice and then infected with VSV for 8 h. b, c Tob1+/+ or Tob1−/− mice (n = 4) were infected with VSV (4 × 107 pfu/mouse) for 8 h. ELISA of IFN-β (b) and IL-6 (c) in serum. d Tob1+/+ or Tob1−/− mice (n = 6) were infected with VSV (4 × 107 pfu/mouse) for 12 h. Quantitative real-time RT-PCR analysis of VSV mRNA in spleen, lung, and liver. e Hematoxylin and eosin staining of lung tissue sections with VSV (4 × 107 pfu/mouse) infection for 18 h (PBS, n = 3 and VSV, n = 3 per condition). Scale bar, 100 μM. f Kaplan–Meier method was used to evaluate the survival curves (n = 10 per condition). All data are shown as the means ± SD. Significance was determined by unpaired two-tailed Student’s t-test: *p < 0.05, **p < 0.01, ***p < 0.001, ns, p > 0.05. Data are shown as a representative result from three independent experiments.

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