Fig. 6: TOB1 enhances deacetylation of Ifnb1 promoter by recruiting HDAC8. | Communications Biology

Fig. 6: TOB1 enhances deacetylation of Ifnb1 promoter by recruiting HDAC8.

From: TOB1 attenuates IRF3-directed antiviral responses by recruiting HDAC8 to specifically suppress IFN-β expression

Fig. 6

a DMSO or PCI-34501 pretreated mouse primary peritoneal macrophages (PMs) were injected with VSV, and then prepared for ChIP-qPCR assay using anti-H3Ac antibody. b Tob1+/+ or Tob1−/− mouse PMs were injected with VSV, and then prepared for ChIP-qPCR assay performed using an anti-H3Ac antibody. c Luciferase activity analysis of HEK293T cells transiently transfected with IFN-β reporter plasmid, followed by treatment with DMSO or PCI-34051 (5 μM), and then infected with VSV. d Lysates from Tob1+/+ or Tob1−/− PMs infected with VSV were subjected to immunoprecipitation with anti-IRF3 antibody followed by western blot analysis with anti-IRF3 or anti-HDAC8 antibody. Proteins in whole-cell lysate were used as positive controls (Input). All data are shown as the means ± SD. Significance was determined by unpaired two-tailed Student’s t-test: **p < 0.01. ***p < 0.001. Data are shown as a representative result from three independent experiments.

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