Fig. 2: Impact of paternal diet on F1 adult-offspring weights and tissue lipid profiles. | Communications Biology

Fig. 2: Impact of paternal diet on F1 adult-offspring weights and tissue lipid profiles.

From: Paternal low protein diet perturbs inter-generational metabolic homeostasis in a tissue-specific manner in mice

Fig. 2

Adult (16 weeks) male and female NN (NPD sperm and NPD seminal plasma), LL (LPD sperm and LPD seminal plasma), NL (NPD sperm and LPD seminal plasma) and LN (LPD sperm and NPD seminal plasma) offspring a body, b liver and c gonadal fat weight. Hepatic d free fatty acid and e triglyceride levels and serum f protein carbonyls and g isoprostane F2α levels. Relative h hepatic, i gonadal fat and j serum abundance of saturated (Sat) mono-unsaturated (Mono) and poly-unsaturated (Poly) lipids. Z scores of differential lipids between NN, LL, NL and LN offspring in k liver, l gonadal fat and m serum. Cer ceramide, DG diglyceride (water-loss product from fragmentation in source), LPC lyso-phosphatidylcholine, LPE lyso-phosphatidylethanolamine, LPG lyso-phosphatidylglycerol, PA phosphatidic acid, PC phosphatidylcholine, PC-P phosphatidylcholine plasminogen, PC-O phosphatidylcholine plasmalogen, PE phosphatidylethanolamine, PI phosphatidylinositol, SM sphingomyelin, TG triglyceride, TGox oxidised triglyceride. Differential serum lipids in n male and o female offspring. N = 20 offspring (10 males and 10 females) per treatment group, sampled from all litters generated. Data are expressed as mean ± SEM (a–j). ∗p < 0.05, ∗∗p < 0.01. Statistical differences were determined using a random effects regression analysis a–g or one-way ANOVA with Bonferoni post hoc correction h–o.

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