Fig. 6: Induction of pathological overstretching in heart slices cultured in CTCM device.

a Representative traces of the air chamber pressure, fluid chamber pressure, and tissue movement measurements verified that the air chamber pressure changes the fluid chamber pressure, which induces a corresponding tissue slice movement. b Representative traces of the percent stretch and stretch rate of normal stretch (orange) and overstretched (blue) tissue slices. c Bar graphs showing quantification of the cycle time (n = 19 slices/group from different pigs), contraction time (n = 18–19 slices/group from different pigs), relaxation time (n = 19 slices/group from different pigs), tissue movement amplitude (n = 14 slices/group from different pigs), peak contraction speed (n = 14 slices/group from different pigs), and peak relaxation speeds (n = 14 (D0), 15 (D6) slices/group from different pigs), Two-tailed Student t-tests revealed no significant difference in any of the parameters, demonstrating that these parameters remained consistent over 6 days of overstretching culture. Error bars are representative of the Mean ± SD.