Fig. 5: Inhibition of miR204 ameliorates fatty acid β-oxidation in liver.

a Degradation of fatty acids. b Western blot analysis of the phosphorylation of AMPK, PGC1α, and PPARα in the liver of IDH2 KO and WT mice fed an HFD, and treated or not with an miR204 inhibitor. β-actin was used as the internal control. c, d Expression of genes related to cholesterol efflux and synthesis, fatty acid synthesis, and fatty acid β-oxidation in the liver of IDH2 KO and WT mice fed an HFD, and treated or not with a miR204 inhibitor, as determined by quantitative real-time PCR (normalized to GAPDH). Data are means ± SEM of three independent experiments (n = 3). *p < 0.05 vs. WT-HFD mice. #p < 0.05 vs. KO-HFD mice.