Fig. 1: Long-read sequencing using activated T cells. | Communications Biology

Fig. 1: Long-read sequencing using activated T cells.

From: Construction of a trio-based structural variation panel utilizing activated T lymphocytes and long-read sequencing technology

Fig. 1

a Scheme of the establishment of activated T cells. T cells were established from CD19-negative cells derived from PBMCs by CD3/CD28 stimulation and stored in liquid nitrogen. After freezing and thawing, the cells were expanded under IL-2 stimulation. b Numbers of cell resources established in the TMM Biobank. Success rates of the establishment processes are also shown. c Cell surface marker profiles of activated T lymphocytes. d Length of genomic DNA as assessed by pulsed-field gel electrophoresis. Genomic DNA isolated from activated T cells was fragmented using a 29-gauge needle and syringe pump. Representative images of five independent samples (from #1 to #5) before (−) and after (+) the fragmentation steps are shown. e Bivariate plot of the read length (x axis) and aligned read quality (y axis) with kernel density estimation. The threshold used to filter low-quality sequence reads (mean quality score of 6) is shown as a dotted line.

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