Fig. 4: Single-cell multiplex flow cytometry analysis identifies murine RMS cell heterogeneity.

a Flow chart of multiplex flow cytometry analysis used to identify different cell populations in NPE and PE RMS. b tSNE density heatmaps of limb and trunk RMS from PE and NPE mice depicting areas of low and high cell number, based on the expression of the different markers (in a). c tSNE graphs of limb and trunk RMS from PE and NPE mice indicating the distribution of CD45neg and CD45pos cells.