Fig. 2: TTTA, GCAA, and GGCG enriched regions compared with GC-rich regions.
From: Contribution of CRISPRable DNA to human complex traits
a–c TTTA, GCAA, and GGCG content of 18,763 segments (excluding 1237 segments with all-N bases), respectively. The genome segments are ordered by decreasing GC content (gray), and the corresponding PAM content is plotted in the same order (blue). The segments left to the vertical dashed line are the top 2000 segments for GC content, and the segments above the horizontal dashed line are the top 2000 segments for the TTTA, GCAA, and GGCG PAM, respectively. The number in the plot shows the number out of the top 2000 PAM segments that are not in the top 2000 GC-rich segments. d Quantile–quantile plot of the segments GC content v.s. PAM content distributions, where the red circles show the top 2000 PAM segments, and the dashed line represents y = x. e Hexbin plot of segments GC v.s. PAM content, where the color represents the count of points in each hexagon. GC and PAM content is normalized to the 0–1 range.
