Fig. 3: Determining optimal PAPS dye and Mn2+ concentrations in LAMP. | Communications Biology

Fig. 3: Determining optimal PAPS dye and Mn2+ concentrations in LAMP.

From: Improved visual detection of DNA amplification using pyridylazophenol metal sensing dyes

Fig. 3

Three concentrations of Bromo-PAPS were tested with 0, 50, 75, or 150 ΌM of Mn2+. The colors of post-LAMP reactions are shown on the top, and the real-time curves on the bottom. The color coding for the real-time curves is the same as that of the labels for Mn2+ concentrations. a 50 ΌM 5-Bromo-PAPS. b 75 ΌM 5-Bromo-PAPS. c 100 ΌM 5-Bromo-PAPS.

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