Fig. 4: PAPS dyes absorbance spectrum shift during LAMP.

a Absorption measured from 360 to 750 nm wavelength in reactions with 75 μM 5-Bromo-PAPS and 100 μM Mn²⁺. Reactions in triplicate with 1 ng of lambda DNA (1ng-1B, 1ng-2B, and 1ng-3B) or NTC controls (NTC-1B, NTC-2B, and NTC-3B). b 75 μM 5-Nitro-PAPS and 100 μM Mn²⁺. Reactions in triplicate with 1 ng of lambda DNA (1ng-1N, 1ng-2N, and 1ng-3N) or NTC controls (NTC-1N, NTC-2N, and NTC-3N). c Relative gain of absorption at 450 and 550 nm in reactions with 75 μM 5-Bromo-PAPS and 75–150 μM Mn²⁺. Positive reactions are graphed as blue circles and NTC as red with average (N = 3) as test label. d Ratio of absorption at 450 nm over 550 nm from reactions with 75 μM 5-Bromo-PAPS and 75–150 μM Mn²⁺.