Table 1 Cryo-EM data collection and modeling statistics.
PRMT5:MEP50:11-2 F | PRMT5:MEP50 | |
|---|---|---|
Data collection and processing | ||
Magnification | 105,000 | 105,000 |
Voltage (kV) | 300 | 300 |
Electron exposure (e−/Å2) | 40 | 40 |
Defocus range (μm) | −0.75 to −3.0 | −0.75 to −3.0 |
Pixel size (Å) | 0.66 | 0.66 |
Symmetry imposed | D2 | D2 |
Initial number of particle images (no.) | 866,240 | 235,210 |
Final number of particle images (no.) | 207,392 | 101,707 |
Map sharpening B factor (Å2) | −68 | −79.7 |
Overall map resolution (Å) | 3.14 | 3.17 |
FSC threshold | 0.143 | 0.143 |
Refinement | ||
Initial model used (PDB code) | Apo PRMT map | Generated in Relion3.0 |
FSC model (0/0.143/0.5) Å | 3.1/3.2/3.4 | 2.9/3.2/3.6 |
Model composition (per asymmetric unit) | ||
Chains | 2 | 2 |
Non-hydrogen atoms | 7384 | 7265 |
Protein residues | 932 | 925 |
Ligands | 2 | 0 |
B factors (Å2) (min/max/mean) | 24.99/75.31/47.74 | 61.67/137.13/86.91 |
R.M.S. deviations | ||
Bond angles (°) | 0.566 | 0.645 |
Bond distances (Å) | 0.005 | 0.007 |
Validation | ||
MolProbity score | 0.78 | 1.05 |
Clash score | 0.69 | 0.89 |
Poor rotamers (%) | 0.00 | 0.00 |
Ramachandran plot | ||
Favored (%) | 97.73 | 97.58 |
Allowed (%) | 2.16 | 1.87 |
Outliers (%) | 0.11 | 0.55 |
EMringer Score | 3.02 | 2.84 |
