Fig. 1: Fluorescence traces of CiHv1-E241C and alanine mutants.
From: Multiple mechanisms contribute to fluorometry signals from the voltage-gated proton channel
Representative fluorescence responses and proton currents to a voltage step from −60 mV to +100 mV recorded from oocytes expressing Ci-Hv1 channels labeled with TAMRA-MTS at the E241C position (a) and different alanine mutants: H179A (b), H188A (c) and H179A H188A (d). Fh is the fluorescence at the holding voltage of −60 mV, ΔFpeak is negative peak fluorescence change during the voltage step, ΔFss (steady state) is change in fluorescence at the end of the voltage step, ΔFtail is maximum change in fluorescence during repolarization after the voltage step and ΔFhook = ΔFtail – ΔFss. The insets show the measured proton current for the corresponding construct. Representative fluorescence traces were selected from N measurements: (a) N = 72, (b) N = 13, (c) N = 8, (d) N = 28.
