Fig. 5: Effect of PC (phosphatidylcholine) on TAMRA-MTS fluorescence.
From: Multiple mechanisms contribute to fluorometry signals from the voltage-gated proton channel
a Effect of 5 mM PC (phosphatidylcholine) on the emission spectrum of TAMRA-MTS in an aqueous solution, in a cuvette (excitation wavelength: 535 nm). b TAMRA-MTS fluorescence in solution in the presence of PC at different concentrations normalized to the control solution (0 mM PC) measured at the wavelength of maximum emission (N = 5). Representative fluorescence responses and proton currents to a voltage step from −60 mV to +100 mV from oocytes labeled with TAMRA-MTS on E241C, expressing the constructs Ci-Hv1 H179A/H188A (c, d, e), H179W/H188W (f, g, h) and H179/H188 (i, j, k). c, f, i panels show the control VCF signals in the absence of PC, before PC application, while the (d, g, j) panels show VCF signal in the presence of 5 mM PC (orange) and after wash (dark grey). Bar charts in panels e, h, k summarize the average PC effects at different concentrations along with washout. Vertical axis shows ΔFtail/Fh values, normalized to the control value (0 mM PC). Error bars represent SEM, *indicates significant difference (*p < 0.05, ***p < 0.001) compared to the control value using one-way ANOVA. (N = 3 at least for e and k; N = 4 for h).
