Fig. 6: Fluorescence traces of heterodimer CiHv1.
From: Multiple mechanisms contribute to fluorometry signals from the voltage-gated proton channel
Representative fluorescence responses to a voltage step from −60 mV to +100 mV from oocytes labeled with TAMRA-MTS on E241C in only one subunit of the dimer. The TAMRA-labeled subunit was paired with a subunit which does not contain labelable Cys but two Trp residues (a, b, c) or two Ala residues (d, e, f) by mixing RNA of E241C subunits (no Trp) with H179W/H188W or H179A/H188A subunits (no Cys) in a 20–80% ratio, respectively. a, d panels show the control VCF signals in the absence of PC, while the (b, e) panels in the presence of 5 mM PC in the extracellular solution from the same oocyte. Bar charts in panel c, f summarize the average PC effects at 5 mM concentration. Vertical axis shows ΔFtail/Fh values, normalized to the control value (0 mM PC). Error bars represent SEM (N ≥ 4).
