Table 1 Cell screening-imaging platforms.
Comparison criteria | CRaft-ID30 | Raft-seq(this paper) | |||
|---|---|---|---|---|---|
a. Imaging constraints | No limitations | Impacted by seq chemistry | Limited by # of usable channels | Optics impacted by raft | Optics impacted by raft |
b. How cells are Collected | NA | NA | FACS | Raft picking | Raft picking |
c. Genotype resolution | Bulk | Single cell | Bulk | Colony | Single cell |
d. Live cell collection | No | No | Medium viability | Possible | Yes |
e. Genotyping method | NA | Specialized | Bulk NGS | Colony NGS | Single-cell NGS |
f. Pooled scalability | Not pooled | Highest | Yes | Yes | Yes |
g. Bottleneck | Physical plates | ISS Technology | Photo activation | Raft picking | Raft picking |
