Table 2 Genotyping primers.

From: Pooled image-base screening of mitochondria with microraft isolation distinguishes pathogenic mitofusin 2 mutations

Name

Sequence (excluding tags, where necessary)

PCR stage

pMFN2.All.F

GCTCTTCTCTCGATGCAACTCT

1

pMFN2.All.R

GCAGGTACTGGTGTGTGAAC

1

pMFN2.1.F

CACATGGCTGAGGTGAATGC

2

pMFN2.1.R

GCAGGAAGCAATTGGTGGTG

2

pMFN2.2.F

CTCAGAGTCCACCCTGATGC

2

pMFN2.2.R

CACTTGAAAGCCTTCTGCGAG

2

RFP.F

GTTCATGCGCTTCAAGGTGC

1, 2

RFP.R

CAAGTAGTCGGGGATGTCGG

1, 2

GFP.F

TGAAGTTCATCTGCACCACCG

1, 2

GFP.R

TCGCCCTCGAACTTCACCTC

1, 2

PRIMPOL.F

GCAACCCAGTTTTGAAACCA

1, 2

PRIMPOL.R

TCGATGTCCAGCTTTCCTCT

1, 2

gRNA.F

CTTGTGGAAAGGACGAAACACC

1, 2

gRNA.R

TTGTGGATGAATACTGCCATTTGT

1, 2

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