Fig. 5: Anergic B cells lacking NDRG1 survive and compete normally in a polyclonal repertoire.
From: NDRG1 is induced by antigen-receptor signaling but dispensable for B and T cell self-tolerance
a Experimental schematic; lethally irradiated WT or sHEL transgenic CD45.1+ recipients were reconstituted with 80:20 mixtures of WT or Ndrg1−/− IgHEL CD45.2+ BM and WT non-tg CD45.1+ BM. b Percentage of total HEL-binding B220+ CD45.1− cells in the BM and spleen of mice in (a). c Percentage of HEL-binding B220+CD45.1− cells labeled with BrdU after one week. In (b, c), dots are individual mice from one experiment with 5–6 mice per group, lines show means and 95% CI error bars. d. Representative flow cytometry plots of B220+CD43− B cells from BM of sHEL mice reconstituted with 50:50 mixtures of WT IgHEL CD45.1+ BM with either WT IgHEL or Ndrg1−/− IgHEL CD45.2+ BM. e Surface IgM expression on B cell populations gated as in (d). f Intracellular Bim quantification in B220+CD19+IgDa+splenocytes from (d). Each point represents individual mice and lines represent means with 95% CI error bars from one experiment (4–5 per group).
